miR-106b regulates milk fat metabolism via ABCA1 in cow mammary epithelial cells
Research on the mechanisms of milk fatty metabolism is of extreme value in improving molecular breeding in dairy cows,as well as their milk quality.Although some studies have shown that microRNA(miRNA)affect lipid metabolism by targeting specific genes,joint analysis of miRNA-mRNA data has revealed little about the underlying mechanisms related to lipid metabolism in the mammary gland of dairy cows.Through high-throughput sequencing and bioinformatics analysis,a total of 233 pairs of negatively associated miRNA/mRNA were obtained.Among them,miRNAs were down-regulated and mRNAs were up-regulated in 162 pairs.Overexpressing miR-106b impaired lipid metabolism while inhibiting miR-106b enhanced lipid metabolism in bovine mammary epithelial cells.Previous experiments measuring TAGs and cholesterol have shown that ABCA1 promotes milk fat metabolism in mammary epithelial cells.In this study,we constructed a miR-106b-ABCA1 regulatory network map that revealed the molecular mechanisms underlying the regulation of milk lipid metabolism by the miR-106b-ABCA1 pathway,providing a theoretical basis for improving milk quality of dairy cows.
miR-106b milk fat metabolism ABCA1 Cow mammary epithelial cells
Zhi Chen Zhangping Yang
College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,PR China Joint International Research Laboratory of Agriculture & Agri-Product Safety,Ministry of Education,Y
国内会议
江苏扬州
英文
117-137
2019-04-01（万方平台首次上网日期，不代表论文的发表时间）