ROS induced GATA4 and GATA6 down-regulation inhibits StAR expression in LPS-treated porcine granulosa-lutein c
LPS is a major endotoxin produced by gram-negative bacteria and exposure to it commonly occurs in animal husbandry.Previous studies have shown that LPS infection disturbs steroidogenesis,including progesterone production,and subsequently decreases animal reproductive performance.However,little information regarding the underlying mechanisms is available thus far.In the present study,an in vitro luteinized porcine granulosa cell model was used to study the underlying molecular mechanisms of LPS treatment.We found that LPS significantly inhibits progesterone production and down regulates the expressions of progesterone synthesis associated genes(StAR,CYP11A1 and 3β-HSD).Furthermore,the levels of ROS were significantly increased in an LPS-dose dependent manner.Moreover,transcriptional factors GATA4 and GATA6,but not NR5A1,were significantly downregulated.Elimination of LPS-stimulated ROS by melatonin or Vitamin C could restore the expressions of GATA4,GATA6 and StAR.In parallel,StAR expression was also inhibited by knockdown of GATA4 and GATA6.Based on these data,we conclude that LPS impairs StAR expression via the ROS induced down-regulation of GATA4 and GATA6.Collectively,these findings provide new insights in the understanding of reproductive losses in animals suffering from bacterial infection and LPS exposure.
LPS ROS progesterone StAR granulosa-lutein cell
Xiaolu Qu Leyan Yan Rihong Guo Hui Li Zhendan Shi
Key Laboratory of Agricultural Engineering in the Middle and Lower Yangtze Region,MOA.Key Laboratory Key Laboratory of Agricultural Engineering in the Middle and Lower Yangtze Region,MOA.Key Laboratory
国内会议
江苏扬州
英文
138-167
2019-04-01（万方平台首次上网日期，不代表论文的发表时间）