Suppression of miR-1197-3p attenuates H2O2-induced apoptosis of goat luteinized granulosa cells via targeting PPARGC1A
Peroxisome proliferator-activated receptor gamma coactivator-1 alpha(PPARGC1A)acts as a powerful coactivator of many transcriptional factors that relate to granulosa cell(GC)apoptosis.In this study,the miRNAs mediating goat follicular atresia and luteinized granulosa cell(LGC)apoptosis induced by hydrogen peroxide(H2O2)via PPARGC1A were investigated.Our results showed that miR-1197-3p targeted PPARGC1A was predicted by bioinformatics algorithm and verified by luciferase reporter assay.In addition,miR-1197-3p promoted goat LGC apoptosis via PPARGC1A through mitochondrial-dependent apoptosis pathway,and these effects could be restored by PPARGC1A overexpression.Moreover,H2O2-induced LGC apoptosis significantly upregulated miR-1197-3p expression and downregulated PPARGC1A level.Pretreatment of miR-1197-3p inhibitor alleviated LGC apoptosis induced by 400 μM H2O2 for 12 h,and preserved the mitochondrial membrane potential by increasing PPARGC1A expression.In conclusion,miR-1197-3p might act as an essential regulator of goat LGC apoptosis potentially via the mitochondrial-dependent apoptosis pathway by targeting PPARGC1A.
goat granulosa cells miR-1197-3p PPARGC1A mitochondrialdependent apoptosis pathway
Guo-Min Zhang Shi-Yu An Zhi-Hai Lei? Feng Wang?
Joint International Research Laboratory of Animal Health and Food Safety,College of Veterinary Medic Jiangsu Livestock Embryo Engineering Laboratory,Nanjing Agricultural University,Nanjing 210095,China Joint International Research Laboratory of Animal Health and Food Safety,College of Veterinary Medic
国内会议
江苏扬州
英文
184-211
2019-04-01（万方平台首次上网日期，不代表论文的发表时间）