Suppression of sirtuin-1 increases IL-6 expression in human airway epithelial cell
Background: A growing number of studies have demonstrated that the activity and expression level of sirtuin-1(SIRT1)are decreased in asthma patients; however,the mechanisms underlying decreased SIRT1 expression and function are still not completely understood.Interleukin(IL)-6 plays important roles in inflammation during allergic asthma.In this study,we examined whether loss of SIRT1 activity regulated the expression of IL-6 and further verified the underlying mechanisms.Methods: The human airway epithelial cell line 16HBE was used to test the effects of the SIRT1 inhibitor(salermide)on expression of IL-6.IL-6 mRNA and protein expression were assessed with real-time polymerase chain reaction(PCR),immunochemistry,and ELISA.Results: We found that inhibition of SIRT1 by SIRT1 inhibitor increased IL-6 mRNA and protein levels in a time-dependent manner,which was accompanied by increased AKT pathway activation in 16HBE cells.Furthermore activation of AKT showed upregulated expression of the IL-6 protein whereas AKT inhibitor(LY294002)significantly inhibited SIRT1 inhibitor-induced IL-6 expression.Conclusion: Our results indicate the potential role of SIRT1 in regulating inflammation by modulation of IL-6 expression in human airway epithelial cell.
human airway epithelial cell ·Sitruin-1 Interleukin-6
zhenhuaNI LinglingTang XiongbiaoWang
Putuo Hospital
国内会议
2017中华医学会呼吸病学年会暨第十八次全国呼吸病学学术会议
福州
英文
1-1
2017-09-20（万方平台首次上网日期，不代表论文的发表时间）