Effects of pachyman in combination with vinorelbine and cisplatin on tumor growth and the expression of EGFR and K-ras in a mouse model of lung cancer
Objectives: To examine the effects of pachyman in combination with vinorelbine and cisplatin on tumor growth and the expression of epidermal growth factor receptor(EGFR)and K-ras in a mouse model of lung cancer induced using the human lung cancer cell line A549,and to investigate the molecular mechanisms underlying the antitumor effects of pachyman.Methods: We recorded the size of the tumor xenografts in mice after treatment with pachyman monotherapy or with pachyman in combination with vinorelbine and cisplatin.We performed immunohistochemical analysis to determine the levels of expression and distribution of EGFR and K-ras in lung cancer tissues.Real-time fluorescence quantitative PCR was used to determine the relative mRNA expression levels of EGFR and K-ras in lung cancer tissues.Results: Vinorelbine and cisplatin significantly decreased the rate of growth of A549 xenografts,and pachyman increased the efficacy of vinorelbine and cisplatin.EGFR and K-ras are widely expressed in A549 xenografts.Vinorelbine and cisplatin can significantly decrease the expression,distribution,and mRNA expression levels of EGFR and K-ras in tumor tissues.Pachyman monotherapy significantly decreased the distribution and the mRNA expression levels of EGFR in lung cancer tissues.In addition,pachyman in combination with vinorelbine and cisplatin markedly decreased the distribution and expression levels of EGFR in lung cancer tissues.However,pachyman monotherapy or combination therapy did not significantly decrease the mRNA expression levels of K-ras.Conclusions: Thus,pachyman in combination with vinorelbine can significantly inhibit the growth of A549 xenografts,and pachyman can regulate the expression of the EGFR gene to increase the efficacy of vinorelbine and cisplatin in lung cancer and decrease the side effects associated with chemotherapy.
Pachyman Lung cancer EGFR K-ras
Ke WEI Jia-hao ZHOU Yong-chao CHEN Jia-ming TIAN Bai-hui LI Ling LI Yi NING Jue HU Qin XIANG Bo ZHANG Fang-guo LU
School of Medicine,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China
国内会议
长沙
英文
184-191
2018-12-29（万方平台首次上网日期，不代表论文的发表时间）