ATP-Citrate Lyase Gene(SoACLA-1),a Novel ACLA Gene in Sugarcane,and Its Overexpression Enhance Drought Tolerance of Transgenic Tobacco
ATP-citrate lyase catalyzes cracking citrate to produce acetyl CoA in the cytoplasm and plays an important role in normal growth and development of plants.In this study,SoACLA-1 gene in sugarcane was cloned and characterized.The amino acid sequence of SoACLA-1 showed high homology with other ACLAs families,and they were clustered together in the phylogenetic tree.On transcript level,SoACLA-1 expressed strongly in root and stalk and responded to polyethylene glycol 6000,drought and ABA stresses.The results indicated that SoACLA-1 possibly involves in stress-responsive metabolic process.PCR analysis was performed to confirm the putative transgenic type in T1 tobacco plants.Overexpression of SoACLA-1 enhanced the tolerance of tobacco plants to drought.The sense transgenic tobacco plants were healthier,had longer roots and higher contents of relative water content and chlorophyll and activities of superoxide dismutase,peroxidase and catalase,but lower ion leakage and contents of malondialdehyde(MDA)and H2O2 than the wild-type and antisense transgenic plants under drought stress.In conclusion,SoACLA-1 plays an important role as a positive factor in drought stress response pathways and can be utilized for transformation to improve crop drought tolerance.
Sugarcane ATP-citrate lyase Overexpression Drought stress Response tolerance Tobacco
Thi-Thu Phan Jian Li Bo Sun Jia-Yi Liu Wen-Hui Zhao Chan Huang Li-Tao Yang Yang-Rui Li
Agricultural College,State Key Laboratory of Conservation and Utilization of Subtropical Agro-biores Agricultural College,State Key Laboratory of Conservation and Utilization of Subtropical Agro-biores
国内会议
广西防城港
英文
1-12
2017-07-01（万方平台首次上网日期，不代表论文的发表时间）