Ionic liquid-regenerated macroporous cellulose monolith:fabrication,characterization and its protein chromatography
Macroporous cellulose monolith as chromatographic support was successfully fabricated from an ionic liquid dissolved cellulose solution by an emulsification method and followed by the cross-linking reaction and DEAE modification.With the physical characterization,the cellulose monolith featured by both the interconnected macropores in range of 0.5-2.5 μm and the diffusion pores centered at about 10 nm.Given the bimodal pore system,the monolith possessed the specific surface area of 36.4 m2 g-1 and the column permeability of about 7.45×10-14 m2.After the DEAE modification,the anion cellulose monolith was evaluated for its chromatography performances.It demonstrated that the static and dynamic adsorption capacity of BSA reached about 66.7 mg mL-1and 43.9 mg mL-1 at 10% breakthrough point,respectively.The results were comparable to other chromatographic adsorbent.In this way,the prepared cellulose monolith with bimodal pores system is expected for the potential application in high-speed chromatography.
cellulose porous monolith emulsification ionic liquid protein chromatography
QIAO Liangzhi DU Kaifeng
Department of Pharmaceutical&Biological Engineering,School of Chemical Engineering,Sichuan University,Chengdu 610065,China
国内会议
天津
英文
364-370
2018-11-18（万方平台首次上网日期，不代表论文的发表时间）