Anintegrated strategy to qualitatively differentiate components of raw and processed Viticis Fructus based on NIR,HPLC and UPLC-MS analysis
Viticis Fructus(VF)is a traditional Chinese herbal drug processed by various methods to achieve different clinical purposes.Thermal treatment potentially alters chemical composition,which may impact on effectiveness and toxicity.In order to interpret the constituent discrepancies of raw versus processed(stir-fried)VF,a multivariatedetection method(NIR,HPLC,and UPLC-MS)based on metabonomics and chemometricswas developed.Firstly,synergy interval partial least squares(siPLS)and partial least squares-discriminated analysis(PLS-DA)was employed to screen the distinctive wavebands(4319-5459cm-1)based on preprocessed NIR spectra.Then,high-performance liquid chromatography(HPLC)with principal component analysis(PCA)was performed to characterize the distinction.Subsequently,a total of 49 compounds were identified by ultra-performance liquid chromatography with mass spectrometry(UPLC-MS),among which 42 compounds were eventually characterized to have the significant change during processing via the semi-quantitative volcano plot analysis.Moreover,based on the PCA-PLS-DA analysis,16 compounds were chosen as characteristic markers which could be in close correlation with the discriminatory NIR wavebands.Together,all of these characterization techniques effectively discriminated raw and processed products of VF.In general,our work provides an integrated way of classifying VF and a strategy to explore discriminatory chemical markers for other traditional Chinese herbs,thus ensuring safety and efficacy for consumers.
Viticis Fructus multivariate statistical analysis quality control Introduction
Jia-Yin Diao Fei Sun Yu Zhang Yue-Wei Ge Shu-Mei Wang
School of Chinese Materia Medica,Guangdong Pharmaceutical University,Guangzhou,China School of Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou, China;Key Laborato
国内会议
江西赣州
英文
312-322
2018-08-01（万方平台首次上网日期，不代表论文的发表时间）