Study on tanshinone Ⅰ and tanshinone ⅡA metabolites in vivo in rats
An efficient method of ultra-high performance liquid chromatography coupled with linear ion trap-Orbitrap(UHPLC-LTQ-Orbitrap)mass spectrometer was established to elucidate the in vivo metabolic process of tanshinone Ⅰ and tanshinone ⅡA in rats.Urine and plasma samples were collected after oral gavage.After the process of biological sample solid phase extraction,the gradient elution was performed using Waters ACQUITY BEH C18 column(2.1 mm×100 mm,1.7 μm,Waters Corporation,Milford,MA,USA)with 0.1%formic acid(A)-acetonitrile(B)solution as the mobile phases.The plasma,urine and the blank samples were detected by ESI-LTQ-Orbitrap equipped with an ESI ion source in positive ion mode.On the basis of the accurate mass measurements,multiple mass spectra and comparison of data with published literature,a total of 26 metabolites were tentatively identified and characterized.Among them,7 metabolites were derived from tanshinone Ⅰ through metabolic pathways of glucuronidation,hydroxylation,hydrogenation,demethylation,methylation,sulfonation,and their composite reactions.19 metabolites were elicited by tanshinone ⅡA through metabolic pathways of hydroxylation,hydrogenation,methylation,sulfonation,glucuronidation,glycosylation,and their complicated reactions.The results comprehensively clarified the metabolism of tanshinone Ⅰ and tanshinoneⅡ A in rats using UHPLC-LTQ-Orbitrap mass spectrometer, which provided material basis for the further research in terms of pharmacodynamics, toxicology, and secondarydevelopment of Chinese medicine.
tanshinone Ⅰ tanshinone ⅡA UHPLC-LTQ-Orbitrap metabolites
ZHAO Wen-jing LIANG Yao-yue WANG Chen-xiao WANG Zi-jian ZHANG Jia-yu WANG Zhi-bin
School of Chinese Pharmacy,Beijing University of Chinese Medicine,Beijing 100102,China Beijing Institution of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China
国内会议
江西赣州
英文
413-428
2018-08-01（万方平台首次上网日期，不代表论文的发表时间）