会议专题

The study of Dried Ginger and Linggan Wuwei Jiangxin decoction against cold asthma rat using GC-MS based metabolomics

  Dried ginger is main components in Linggan Wuwei Jiangxin decoction,which both treat cold asthma in clinic.The study was to explore and compare the effects of dried ginger and Linggan Wuwei Jiangxin decoction against cold asthma at metabolomics level using GC-MS,which provides a basis for the treatment of cold asthma with dried ginger and Linggan Wuwei Jiangxin decoction and gives some references for studying Chinese Medicine compatibility.A cold asthma rats model was established firstly,then general morphological observation,hematoxylin eosin staining,inflammatory cell count and Ig-E,IL-4,IFN-γ and visceral index were chosen to assess the model.The metabolomics based on GC-MS with multivariate statistical analysis was performed.Biomarker identification,pathways analysis,correlation relationship between identified biomarker and efficacy indexes were carried out successively.Dried ginger and Linggan Wuwei Jiangxin decoction have obvious effects on cold asthma.Total 37 metabolites(15 in serum and 22 in urine)possibly contributed to the progress of cold asthma were identified.These metabolites mainly involve carbohydrate,fatty acids and their products,organic acid and others.Combined with pathway analysis,there involved in seven pathways.After intervention by dried ginger and Linggan Wuwei Jiangxin decoction,the majority of altered metabolites and metabolic pathway were effectively reversed,and Linggan Wuwei Jiangxin decoction had powerful effect on pathways to energy metabolism and oxidative stress than dried ginger against cold asthma.It was concluded that dried ginger and Linggan WuWei Jiangxin decoction both produced the beneficial effects on cold asthma.And from metabolites level,Linggan Wuwei Jiangxin decoction regulated more metabolite of carbohydrate and fatty acid which contribute to energy metabolism and oxidative stress in cold asthma than these of dried ginger,which possibly related to the interaction of various herbs and complex chemical components in Linggan Wuwei Jiangxin decoction.In addition,this study provides a reference for the research on the compatibility of Chinese Medicine.

dried ginger linggan wuwei jiangxin decoction cold asthma metabonomics compatibility

Shan Ran Yanquan Han

The First Affiliated Hospital of Anhui University of Chinese Medicine,Anhui,Hefei,230000,China

国内会议

第十五届长三角科技论坛——2018年长三角色谱分析技术共建共享创新成果学术研讨会

合肥

英文

30-57

2018-11-03(万方平台首次上网日期,不代表论文的发表时间)