Sweroside from the flowers of Gentiana macrophylla Pall.ameliorate collagen-induced arthritis in rats
OBJECTIVE:This work aimed to investigate the anti-rheumatoid arthritic effect and possible mechanism of iridoid glycosides from G.macrophylla(SWE)using an animal model of collagen-induced rheumatoid arthritis(CIA)in rats.METHODS:All rats were randomly divided into five groups:normal control,CIA,dexamethasone,15 mg/kg and 30 mg/kg SWE.The administration of different drugs started from day 1 and continued for 28 days.Paw swelling,arthritis score and histopathological changes were examined to assess the severity of arthritis.In addition,the serum levels of tumor necrosis factor α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6),and cyclooxygenase-2(COX-2)and inducible nitric oxide synthase(iNOS)expressions in joint synovial tissues were detected.RESULTS:SWE reduced paw edema,arthritis scores and the index of spleen and thymus from day 7 and 21 after CIA compared with those in the CIA group.Our date also demonstrated that SWE inhibited pro-inflammatory cytokines such as TNF-α,IL-1β and IL-6,regulating the expression of iNOS and COX-2 compared with those in the CIA group.CONCLUSION:Taken together,our results demonstrated that SWE possessed potential therapeutic effect on collagen-induced arthritis in rats by reducing the levels of IL-1β,IL-6 and TNF-α in serum as well as down-regulating the levels of iNOS and COX-2,and therefore may be an effective therapy for the treatment of rheumatoid arthritis.
Sweroside Gentiana macrophylla Pall. rheumatoid arthritis collagen-induced arthritis
Na Jia Jia Cui Aidong Wen
Department of Pharmacy,Xijing Hospital,Fourth Military Medical University,Xi'an 710032,PR China
国内会议
杭州
英文
89-97
2018-07-03（万方平台首次上网日期，不代表论文的发表时间）