Involvement of slr0081, a Two-Component Signal-Transduction System Response Regulator,in Acid Stress Tolerance in Synechocystis sp. PCC 6803
Two-component signal transduction is the primary signaling mechanism used for global regulation of cell response to changes in the environment. DNA microarray analysis identified genes up-regulated by acid stress in cyanobacteria Synechocystis sp. PCC 6803. Several of these altered genes are thought to be response regulators that are directly involved in this type of stress. Deletion mutants of response regulator genes were constructed and survivability was compared between the cells transfected with mutant and wild-type genes in a low-pH medium. Among these, deletion of slr0081 affected the growth rate under conditions of acid stress (pH 6.0). We examined the genome-wide expression of genes in Aslr0081 mutant cells by using DNA microarray in an attempt to determine whether slr0081 is involved in the regulation of other acid stress responsive genes. Our findings by quantitative real-time RT PCR revealed that down regulation of acid responsive genes slr0967 and sll0939 occurs by deletion of slr0081.
Low-pH Cyanobacteria Stress response Two-component system
Yu Tanaka Mayuko Kimura Atsushi Moriyama Yuko Kubo Mamoru Sambe Junji Uchiyama Hisataka Ohta
Department of biology, Faculty of Science, Tokyo University of Science, Shinjyuku, Tokyo 162-8601, J Research center for RNA science, RIST, Tokyo University of Science, Noda, Chiba 278-8510, Japan Department of biology, Faculty of Science, Tokyo University of Science, Shinjyuku, Tokyo 162-8601, J
国际会议
15th International Conference on Photosynthesis(第15届国际光合作用大会)
北京
英文
593-595
2010-08-22（万方平台首次上网日期，不代表论文的发表时间）