IMMUNOHISTOLOGY OF POPLAR STEM REVEALS THAT MANNAN POLYSACCHARIDES ARE ACCUMULATED DURING XYLEM DEVELOPMENT

摘要：

Mannan polysaccharides serve as storage reserves in seeds and as structure elements in cell walls, and they may also perform other important functions during plant growth. As one of the major hemicelluloses in wood of angiosperm, little is known about the presence and localization of mannan polysaccharides during xylem development in the model tree, Populus trichocarpa. In this study, we use mannan-specific recognized antibody to label mannan polysaccharides in stem tissues of different developmental stage. Immunofluorescence microscopy showed that the epitopes were localized in xylem elements, especially in thickened secondary cell walls and interfascicular fibers, and the signals are consistent with lignification. Other cell types reveal low level of mannan epitopes. These results demonstrate that mannans are particular significance in lignified cell walls in xylem tissue, which provide us a further opportunity to study biosynthesis of mannans during xylem development in wood.

关键词： carbohydrate-binding module (CBM) immunofluorescence mannan polysaccharides monoclonal antibody plant cell walls

作者: Hsin-Tzu Wang I-Hsuan Liu Ting-Feng Yen

作者单位: School of Forestry and Resource Conservation, National Taiwan University, Taipei 10617, Taiwan Department of Animal Science and Technology,National Taiwan University, Taipei 10617, Taiwan

会议类型: 国际会议

会议名称: 16th International Symposium on Wood,Fiber and Pulping Chemistry(第十六届木材、纤维及制浆化学国际会议)

会议地点: 天津

会议语种:英文

页码: 56-59

在线出版日期: 2011-06-08（万方平台首次上网日期，不代表论文的发表时间）