Analysis of Longan POD Isoenzyme Activity
The methods of POD isoenzyme extraction and electrophoresis were optimized.The result showed that 3 to 6 month young leaf was suitable for POD isoenzyme.The extraction buffer contains 0.05 mol/L phosphate buffer (Ph 7.5) and 0.1％ TritonX-100. POD isoenzyme was separated by polyacrylamide gel electrophoresis. The zymogram of POD isoenzyme increased from 8 to 17 using improved POD extraction and electrophoresis methods.The zymogram was divided into 4 regions. Region Ⅰ and Ⅲ were active regions. The zymograms in these regions were active and conserved.Region Ⅱ and Ⅳ were weak regions,and the zymograms were weak. It was concluded that the second and third bands were distinct bands.
PAGE POD extraction zymogram relative modification
F.L.Zhong Z.X.Guo T.L.Liu C.Y.Ma D.M.Pan
College of Horticulture Institute of Storage Science and Technology of Horticultural Products Fujian Agricultural and Forestry University Fuzhou 350002,Fujian China
国际会议
福州
英文
315-321
2008-08-25（万方平台首次上网日期，不代表论文的发表时间）