Electrochemical Detection of DNA Species Related to Cymbidium Mosaic Virus Based on Site-specific DNA Cleavage of Restriction Endonuclease and Using Quantum Dots as Reporter
Cymbidium mosaic virus (CymMV) is one of the most prevalent and important orchid viruses 1. It infects numerous commercially important orchid genera and has attained a worldwide distribution 2. CymMV cause flower colour breaking, size reduction and stunted growth, thus reducing the quality of the orchids and affecting of the orchid industry. Consequently, some techniques have been employed for detection of CymMV. Such as, visual observations, indirect enzyme-linked immunosorbent assay (ELISA), real-time quantitative reverse transcription PCR (RT-PCR) 3. But there were some limitations in these methods, such as time-consuming, poor precision and expensiveness. So it is very significant to develop a new effective method. Within recent years, several designs for virus sensors based on an electrochemical readout have appeared due to the fact that electrochemical detections are simple, reliable, cheap, sensitive, and selective for genetic detection 4.
Jinghua CHEN Jing ZHANG Guonan CHEN
The Key Lab of Analysis and Detection Technology for Food Safety of the MOE, Department of Chemistry The Key Lab of Analysis and Detection Technology for Food Safety of the MOE, Department of Chemistry The Key Lab of Analysis and Detection Technology for Food Safety of the MOE, Department of Chemistry
国际会议
The Twelfth International Symposium on Electroanalytical Chemistry(第十二届国际电分析化学会议 12th ISEC)
长春
英文
139-140
2009-08-12（万方平台首次上网日期，不代表论文的发表时间）