Electrochemical Detection of DNA Hybridization Based on Bio-bar Code Method
Sequence-specific detection of DNA targets has become increasingly important for the diagnosis and treatment of genetic diseases, and forensic analysis 1, 2. In the DNA detection processes, the amplification steps are important to realize the ultimate in terms of sensitivity. These amplification systems include polymerase chain reaction (PCR) protocols, and signal-amplification systems, such as fluorogenic substrate-active enzymes, modified lipsomes, and nanoparticles 3,4.The bio-bar code amplification assay created by Mirkin et. al. is the only biodetection method that has the PCR-like sensitivity for both protein and nucleic acid targets without a need for enzymatic amplification 5, 6. The typical bio-bar code assay has been described in many reports. The assay has exhibited low-attomolar sensitivity for a variety of protein targets and high-zeptomolar sensitivity for nucleic acid targets when paired with scanometric readout.
Caifeng DING Qian ZHANG Jin-Ming LIN
The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, T Key Laboratory of Eco-chemical Engineering, Ministry of Education College of Chemistry and Molecular The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, T
国际会议
The Twelfth International Symposium on Electroanalytical Chemistry(第十二届国际电分析化学会议 12th ISEC)
长春
英文
159-160
2009-08-12（万方平台首次上网日期，不代表论文的发表时间）