Proteomics Study of COI1-regulated Proteins in Arabidopsis Flower
Jasmonates (JAs) are a new class of plant hormone that regulate expression of diverse genes to mediate various plant responses. The Arabidopsis F-box protein COM is required for plant defense and male fertility in JA signal pathway. To further investigate the regulatory role of COM in male fertility, we compared the proteomics profiles of Arabidopsis wild type (WT) flowers with coi1-1 mutant male-sterile flowers using two-dimensional difference gel electrophoresis coupled with matrix-assisted laser desoption/ionization-time-of-flight mass spectrometry. Sixteen proteins with potential function in specific biological processes such as metabolism processes and defense/stress responses were differentially expressed in WT and coi1-1 mutant flowers. Verification on a phi class glutathione transferase AtGSTF9, one out of these 16 identified proteins, revealed that the expression of AtGSTF9 was severely downregulated in flowers of coi1-1 mutant compared with that of WT. Further function analyses of these genes would provide new insights into the molecular basis of COI1-regulated male fertility.
Lingling Chua Xiaoyi Shan Junxia Wang Wen Peng Guiyou Zhang Daoxin Xie
Institute of Molecular and Cell Biology, 61 Biopolls Drive, Singapore 138673 Protein Science Laboratory of the Ministry of Education, School of Life Sciences, Tsinghua Universit Institute of Molecular and Cell Biology, 61 Biopolls Drive, Singapore 138673;Protein Science Laborat
国际会议
2009整合植物生物学研讨会(Integrative Plant Biology Symposium)
烟台
英文
410-419
2009-08-10（万方平台首次上网日期，不代表论文的发表时间）