Environmental Stress Tolerance and Field Performance of Transgenic Tall Fescue Overezpressing NDPK2 Gene
Environmental stress is a growing problem for the productivity of forage crops. Reactive oxygen species (ROS) formation due to several abiotic stresses is a fundamental process that interrupts several physiological processes and causes a significant reduction on growth and yield of many forage crops. Molecular breeding such as genetic transformation has become a popular biotechnological tool for improving forage quality as well as improves tolerance to various abiotic stresses. As a first step of genetic transformation in tall fescue, we established an efficient Agrobacteriunrmediated genetic transformation protocol using mature seed-derived embryogenic callus. After optimization of the transformation system, several genes of interest have been used to generate abiotic stress tolerant forage grasses. We generated transgenic tall fescue plants expressing NDPK genes under the control of the oxidative stress-inducible SWPA2 promoter. Transgenic plants showed enhanced tolerance to several abiotic stresses. Results in the current study, suggest that NDPK mediated multiple stress tolerance by increasing the expression of genes involved in antioxidant and protective functions, possibly through activation of an MAPK cascade.
Forage crops Tall fescue Transgenic plant AtNDPK2
Sang-Hoon Lee Ki-Won Lee Ki-Yong Kim Sang-Soo Kwak Byung-Hyun Lee
Grassland & Forages Research Center, National Institute of Animal Science, RDA, 330-801, Korea Grassland & Forages Research Center, National Institute of Animal Science, RDA, 330-801, Korea Divis Laboratory of Environmental Biotechnology, KRIBB, Daejeon 305-806, Korea Division of Applied Life Science, Gyeongsang National University, Jinju 660-701, Korea
国际会议
首尔
英文
140-141
2009-08-10（万方平台首次上网日期，不代表论文的发表时间）