Warm-water Emasculation in Self-pollinating Buckwheat
A simple and practical method to replace hand emasculation is necessary for routine breeding in small flowered crops such as buckwheat. Three step by step experiments were conducted, using warm-water emasculation in self-pollinating buckwheat.High temperature impeded pollen function in mature buds that opened the day following the treatments, rather than in the young buds that did not open the next day but opened in the following week. The treatment at 44℃for 3 min did not completely disable pollen function that resulted in an average of 41.54%(15.38%-56.25%)seed set by selfing.Applicable temperatures combined with time duration were achieved through either increasing the time by one min i.e.at 44℃for 4 min or by raising the temperature one degree i.e.at 45℃for 3 min. Theformer method had a selfed seed set of 1.5%(2 seeds out of 148 buds), however there was no seed set at 45℃for 3 min.Hand pollination produced an average of over 65%seed set after warm-water emasculation at 44℃for 4 min and at 45℃for 3 min, which Was comparable to hand emasculation, however, with a large saving of labor and time.
Warm-water emasculation Temperature Self-pollinating Buckwheat Breeding
Yingjie Wang Clayton G.Campbell
Kade Research Ltd., Morden, MB.Canada
国际会议
The 10th International Symposium on Buckwheat(第十届国际荞麦会议)
陕西杨凌
英文
100-104
2007-08-14(万方平台首次上网日期,不代表论文的发表时间)