Micro-Electrode Cell-Based Biosensor Using Electrochemical Impedance Spectroscopy for Cancer Research

摘要：

In this study,cell biosensor chip with microelectrode arrays was used to monitor the culture behaviour of mammalian cancer cells and evaluate the chemosensitivity of anti-cancer drugs using electrochemical impedance spectroscopy.The electrode arrays were fabricated by semiconductor technology with diameter from 50 μm to 10 μm of each electrode.The human oesophageal cancer cell lines (KYSE 30) were cultured on the surface of the electrode with the help of fibronectin.Morphology changes of cells adhesion,spreading,and proliferation can be detected by impedimetric analysis in a real time and non-invasive way.Anti-caner drug of cisplatin was added to cells for potential drug screening applications.The experimental results show that drug’s chemosensitivity effects can be detected by this cell based biosensor.

关键词： Cell-based biosensor Micro-electrode arrays Cancer Impedance spectroscopy Chemosensitivity Anticancer drugs

作者: Qingjun Liu Jinjiang Yu Hui Yu Lidan Xiao Ping Wang Mo Yang

作者单位: Biosensor National Special Laboratory,Department of Biomedical Engineering,Zhejiang University,Hangz Department of Health Technology and Informatics,The Hong Kong Polytechnic University,Kowloon,Hong Ko

会议类型: 国际会议

会议名称: 7th Asian-Pacific Conference on Medical and Biological Engineering(第七届亚太地区生物工程学术会议)

会议地点: 北京

会议语种:英文

在线出版日期: 2008-04-22（万方平台首次上网日期，不代表论文的发表时间）