Dynamic expression of rat heat shock protein gp96 and its gene during development of hepatocellular carcinoma
Hepatocellular carcinoma (HCC) is characterized by multicause, obvious multistage and multifocus process of tumor progression. Development of HCC is related intimately with overexpression and signal transduction of many cellular factors. The aim of this study was to investigate the dynamic expression and alteration of heat shock protein (HSP) gp96 along with its gene during HCC development. Rat hepatoma models were established by using male S-D rats induced with 2-Fluorenylacetamide. Total RNAs were transcripted into cDNA by reverse transcription and the gene fragment of gp96 was amplified by nested RT-PCR. The gp96 expressions in rat liver tissues were semi-quantitatively analyzed by immunohistochemistry. An increasing tendency of liver gp96 protein was found from normal liver to precancerous to cancerous tissues during rat hepatoma development (P<0.01), which was in accordance with gp96 mRNA alteration (P<0.05). HSP gp96 is involved in HCC development and its overexpression could be useful marker for early diagnosis of HCC.
hepatocellular carcinoma HSPgp96 RT-PCR immunohistochemistry
Xin-Hua Wu Deng-Fu Yao Xiao-Qin SU Bo-Jun Tai Hua Huang Li-Wei Qiu Wei Wu Yi-Xiang Shao
Research Center of Clinical Molecular Biology;Department of Infectious Diseases Research Center of Clinical Molecular Biology Department of Infectious Diseases Department of Pathology, the Affiliated Hospital of Nantong University The Center of Medical Laboratory Animals, Nantong University, Nantong，226001, China
国际会议
The 5th International Forum on Post-genome Technologies(5IFPT)(第五届国际后基因组生命科学技术学术论坛)
苏州
英文
2007-09-10（万方平台首次上网日期，不代表论文的发表时间）