Prev e nt reconstruction of ischemic myocardium with autologous stem cells transfected by AKT gene
Background: To test the hypothesis that AKT1-engineered Mesenchymal stem cells (MSCs) aremore resistant to apoptosis than MSCs and can enhance cardiac repair after transplantation into theischemia swine heart.Methods :All ischemia swines were divided into four groups: the control group(A), the DMEMgroup (B), the MSCs group(C), and the AKT-transfected group(D). The MSCs were transfectedwith the AKT1 gene, after which autologous BrdU labeled stem cells was injected into LAD of theinfarct heart by transcatheter in the group C and D. In the group B, DMEM was injected in thesame distraction by the same approach. In group A, there is no other injection after occluding theLAD. Then 4 weeks later, the cardiac function and regional perfusion measurements wererepeated by MRI and Echocardiography, and the histological characteristics of the hearts were alsostudied to assess MSCs engraftment. VEGF, TGF-β 1, Connecxin-43, BrdU and VWF were tested.Results: The cardiac function was significantly improved after the cells implantation, and theimplanted MSCs prevented the infarct region from thinning and expanding, improved contractionand increased perfusion in all groups relative to the control group. Moreover, the improvementwas even markedly greater in Group D.Conclusion The MSCs engraftment in host myocardium might improve the cardiac function byattenuating the contractile dysfunction and pathologic thinning in this model of left ventricularwall infarction.
YU Yunsheng SHEN Zhenya YE Wenxue CHEN Yihuan LAO Weijie TAO Li
the department of cardiovascular surgery ,the first affiliated hospital of soochow university Suzhou china 215006
国际会议
成都
英文
1-7
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）