Biotransformation of sodium L-glutamate to γ -aminobutyric acid by L. brevis TCCC13007 with two glutamate decarboxylase genes
Due to the important physiological functions of GABA,various methods used to produce it. Biotransformation bymicroorganism cells is a more promising method than others.Among the GABA-producing lactic acid bacteria, one strain withhigh producing capacity was isolated from Chinese naturallypickled vegetable. The strain is identified as Lactobacillus brevis,and named as L.brevis TCCC13007 based on its physiological andbiochemical characteristics and homology of 16S rDNA. In thisstudy, two gad genes were cloned by PCR using the genomic DNAof L.brevis TCCC13007 as template. The deduced amino acidsequence of one gad gene showed 100%, 98.5% and 95.9%identity of L.brevis ATCC367, L. brevis BH2 and L. brevis OPK-3,respectively. The other showed 99.8% and 99.4% identity ofL.brevis ATCC367 and L.brevis IFO12005respectively. Accordingto the properties of GAD, the optimal biotransformationcondition was investigated. Under the optimal condition, L.brevisTCCC13007 synthesised GABA at a concentration of 39 g.L -1.
Ying Zhang Nian-fa Gao Yu Feng Lei Song Qiang Gao
Key Laboratory of Industrial Microbiology Ministry of Education, School of Biotechnology, Tianjin Un Key Laboratory of Industrial Microbiology Ministry of Education, School of Biotechnology, Tianjin Un
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）