Optimization of Fermentation Parameters for a-Amylase by Bacillus subtilis ZJF-1A5 with Corn Dextrin as Carbon Source
Fermentation Parameters produced a-Amylase by Bacillus, subtilis ZJF-1A5 was investigated using dextrin with different DE value as carbon source. The results showed that yield and activity of α-amylase were relatively high when the DE value of dextrin was controlled in the range of 16-22 and inoculation amount of B. subtilis ZJF-1A5 was 3％-4％ (v/v). The shake flask fermentation parameters were temperature 37℃, pH 5.0, shaker speed 210 r/min and the culture time 54h. Compared with starch and glucose, dextrins with DE value 16-22 were used as carbon source, the activities of a-amylase increased by 80.2％ and 219.3％, respectively.
α-amylase bacillus subtilis corn dextrin fermentation
SUN Junliang ZHAO Ruixiang LIANG Xinhong ZENG Jie
School of Food Science, Henan Institute of Science and Technology, Xinxiang, Henan Province, P.R.China, 453003
国际会议
威海
英文
396-401
2010-07-24（万方平台首次上网日期，不代表论文的发表时间）