Selenoprotein Synthesis and Reactivity - Biotechnological and Biomedical Applications

摘要：

Selenoproteins contain the chemically reactive selenium-containing amino acid selenocysteine (Sec),which is co-translationally incorporated at a UGA (opal) codon via intricate and unique Secdedicated synthesis machineries.Using a genetic tailoring methodology that enables directed Secinsertion at a pre-defined UGA codon,recombinant selenoproteins can be produced in E.coli (Arner,2002). This technique may be employed for studies of selenoprotein features and detailed analyses of native selenoprotein kinetics,but may also be utilized for the development of new biomedical applications based upon the use of Sec reactivity (Johansson et al.,2005). This presentation will be a short survey of examples utilizing recombinant selenoproteins for in-depth studies and for the development of novel technologies within nuclear medicine.

作者: E.S.J.Arner

作者单位: Division of Biochemistry,Department of Medical Biochemistry and Biophysics,Karolinska Instituted SE-171 77 Stockholm,Sweden

会议类型: 国际会议

会议名称: The First International Conference on Selenium in the Environment and Human Health(第一届硒与环境和人体健康国际会议)

会议地点: 苏州

会议语种:英文

页码: 49-50

在线出版日期: 2009-10-01（万方平台首次上网日期，不代表论文的发表时间）