Transformation and Construction of RNAi Ezpression Vector of Plant Trehalase Gene
The trehalase (TRE) is one of enzymes related to trehalose catabolism in plant. On the basis of previous experiments, we are trying to inhibit the expression of trehalase gene through RNA interference technology in order to achieve the purpose of increasing the accumulation of trehalose in plant. So the fragments of TRE gene and RNAi-TRE have been obtained from Arabidopsis seedling by using RT-PCR (reverse transcription PCR) method, then they were constructed into pBI121 (plant expression vector) and pGSA1285 (RNAi expression vector) respectively. After transformed to tobacco, the expression of TRE gene has been detected through real-time quantitative PCR in transgenic tobacco. The result shows that the mRNA level of TRE gene decreased obviously in transgenic tobacco which has been transformed with RNAi-TRE. It indicated that RNA interference technology is also effective to Heterogeneous plant in regulation of gene expression.
trehalase RNAi ezpression vector construction
Guo Bei Jiang Xiang-ning
dept.of biotechnology Beijing University of Agriculture Beijing,China Jiang Xiang-ning college of life sciences and biotechnology Beijing Forestry University Beijing,Chin
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）