A study on the fabrication of porous scaffold crosslinked with genipin
This study was aiming to investigate the fabrication and characterization of the porous chitosan/gelatin scaffold cross-linked with genipin (GP) which is widely used as a natural and non-toxic cross-linking agent. The chitosan/gelatin scaffold was prepared and cross-linked with 1％ or 2％(w/w) genipin for 24 hours at 37°C, then pre-freezing for 48h at -20°C and -80°C before freeze drying. As a control, a chemical cross-linking agent glutaraldehyde (GTA) was investigated simultaneously. The swelling ratio, degradation ratio was determined by measuring change in mass between dry and wet scaffold, and measuring the change of weight in 9-days biodegradable test, respectively. Scanning electron microscopy (SEM) was used to observe the scaffold morphology. The XTT assay was applied to evaluate the cytotoxicity and biocompatibility of the chitosan/gelatin scaffold with MC3T3-E1 cells in this study.
Chitosan Gelatin Genipin Scaffold Crosslinking Cytotozicity Biocompatibility
Jun-Sheng Tian Yuan-Lu Cui Kang-De Yao
Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae,Ministry of Education Tianjin Research Institute of Polymeric Materials Tianjin University Tianjin,Peoples Republic of China,3000
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）