Novel Affinity Membrane Chromatography Technology: Purification of Pepsin Using Immobilized Reactive Dye Ligands

摘要：

Novel membranes were prepared through covalently coupling of chitosan (CS) to activate nylon membrane after the reaction of the microporous nylon membrane with formaldehyde. Non-specific adsorption on the CS-coated nylon membrane decreased greatly. Yellow 3G-P/Reactive Red 120 were then covalently immobilized onto composite membrane as dye ligands. These Red 120/Yellow 3G-P-CS-membranes were used in the Pepsin adsorption studies. According to the results, it appears that Yellow 3G-P-CS-nylon membranes could be applied for separation and purification of Pepsin without causing any denaturation, much better than Red 120-CS-nylon membrane.

关键词： affinity chromatography pepsin adsorption chitosan-nylon membrane

作者: Tian-Xiang Chen Shu-Bai Li Hua-Li Nie Li-Min Zhu Christopher J Branford-White

作者单位: College of Chemistry,Chemical Engineering and Biotechnology,Donghua University,Shanghai,P.R.China Institute for Health Research and Policy London Metropolitan University London,UK

会议类型: 国际会议

会议名称: The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)

会议地点: 北京

会议语种:英文

页码: 1-4

在线出版日期: 2009-06-11（万方平台首次上网日期，不代表论文的发表时间）