Novel Optical Nanobiosensor Based on Localized Surface Plasmon Resonance Assembled with Gold Colloid Nanoparticles
A novel optical biosensor assembled with gold colloidal nanoparticles on gold plate surface has been constructed via 1,4-dithiothreitol and L-cysteic acid for detection of bio-molecules like antigen, antibody protein and DNA plasmid, which exhibited distinct optical properties of localized surface plasmon resonance (LSPR). The prepared nano gold sol were characterized by using UV-Vis absorption spectroscopy, transmission electron microscopy (TEM), and cyclic voltammetry (CV), indicating that the Au colloidals were sphere-shaped and narrow-dispersed size distribution with a diameter of 24 ± 0.2 nm. It caused 2.80 nm of red shift in the peak wavelength (λmax) of LSPR spectrum for the nanosensor exposed to c-Myc (3C7) mouse monoclonal IgG and 3.36 nm of red shift to the corresponding antibody by the immuno-reaction subsequently. The LSPR nanobiosensor has been successfully applied to recognition of a DNA plasmid of pCMV-Myc Mammalian Expression Vector.
optical nanobiosensor localized surface plasmon resonance gold nanoparticles biomolecule recognition
Ling Zhang Gui-Fu Ma Yu Cao Ning Gu Zhong Cao Xun Li Ming Tu Meng-Xue Zeng Xi-Xi Huang Su-Li Guo Mei-Li Chi Chuan-Qi Chen
School of Chemistry and Biological Engineering,Changsha University of Science and Technology,Changsh Jiangsu Laboratory for Biomaterials and Devices,National Laboratory of Molecular and Biomolecular El School of Chemistry and Biological Engineering,Changsha University of Science and Technology,Changsh
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）