Long Term Sensibility of the Ni-Co Protein Chip

摘要：

For the metal ion affinity chromatography, the nickel is used to capture the histidines(his)-tagged protein to make up a rapid purification system from the organism lysate. In this study, based on the optimization approach of Taguchi method, we developed a Ni-Co protein chip whose intermediate metal surface could capture the recombinant protein successfully. Thus, the proposed Ni-Co chip can provide specific binding with His-tagged biotin and His-tagged Uricase. In addition, under the constant humidity and temperature, the immobilization capability of Ni-Co chip with specific binding of immunoreaction was tested every week. Finally, we obtained the long term performance of Ni-Co chip with 6×His-Uricase of concentration of 20 μg/ml.

关键词： transition metal ion affinity chromatography histidines(his)-tagged Biotin Uricase

作者: Yaw-Jen Chang Yeon-Pun Chang Cheng-Hao Chang Chih-Yu Hu

作者单位: Department of Mechanical Engineering Chung Yuan Christian University 200,Chung Pei Rd.,Chung Li,Taiwan 32023,R.O.C

会议类型: 国际会议

会议名称: The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)

会议地点: 北京

会议语种:英文

页码: 1-4

在线出版日期: 2009-06-11（万方平台首次上网日期，不代表论文的发表时间）