Induction of Apoptosis in HepG2 Cells by Solanine
The nightshade(Solanum nigrum Linn.) has been widely used in Chinese traditional medicine as a remedy for the treatment of digestive system cancer. The anti-tumor activity of solanine, a steroid alkaloid isolated from the nightshade has been demonstrated. To observe the effect of antitumor and mechanism of solanine. The MTT assay was used to evaluate the IC50 on the 3 digestive system tumor cell lines; The effect on the morphology was observed with a laser confocal microscopy; The rate of apoptosis and the cell cycle were measured using flow cytometry. The results show the IC50 for HepG2, SGC-7901, and LS-174 were 14.47, >50, and >50μg/ml, respectively; The morphology of cells in the negative control was normal; For the treated groups, typical signs for apoptosis were found. The rate of apoptosis in HepG2 cells induced by solanine was found to be 6.0％, 14.4％, 17.3％, 18.9％, and 32.2％, respectively. Observation of the cell cycle showed that cells in the G2/M phases disappeared while the number of cells in the S phase increased significantly for treated groups. Therefore, the solanine could induce apoptosis in HepG2 cells.
solanine HepG2 apoptosis
Shiyong Gao Yubin Ji Chenfeng Ji Xiang Zou
Postdoctoral Programme of the Institute of Materia Medica,The Center for Life Sciences and Environme MOE Engineering Research Center of Natural Anticancer Drugs Harbin Commerce University Harbin,China
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）