Application of Agilent 2100 Bioanalyzer in detection of foodborne pathogens Salmonella
To explore the feasibility of using Agilent 2100 Bioanalyzer in the detection of foodborne pathogens Salmonella spp. The invA gene of Salmonella spp. was amplified by PCR technique using specific primers. Agilent 2100 Bioanalyzer and conventional agarose gel electrophoresis were employed respectively to detection the PCR products. The accuracy, reproducibility and sensitivity of those two detection techniques were compared. Agilent 2100 Bioanalyzer showed more accurate in determining the length of the gene fragments than agarose gel electrophoresis, the accuracy of the Bioanalyzer reached above 95％ while the agarose gel electrophoresis was only 85％. Bioanalyzer was also more sensitive than agarose gel electrophoresis. Agilent 2100 Bioanalyzer combined with PCR is specific, accurate and sensitive for Salmonella detection in the clinical diagnosis.
foodborne pathogens Salmonella Polymerase chain reaction Agilent 2100 Bioanalyzer agarose gel electrophoresis
JING Jian-zhou ZHAO Pei-pei WANG Yun-long BAI Yan-hong
Zheng Zhou University of Light Industry,Zhengzhou Henan,450002 Henan Bioengineering technology research center,Zhengzhou,Henan,450002,P.R.China
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）