BOD BIOSENSOR USING SALT-TOLERANT Bacillus licheniformis FOR SEA WATER
The Possibility of sensing seawater BOD (Biochemical Oxygen Demand) by microbial sensors using tamed Bacillus licheniformis is test.Microbial biosensors of BOD are fabricated based on the Clark oxygen electrode and the biological sensing material is immobilized Bacillus licheniformis isolated from starch wastewater and tamed in saline water.Results show sensors has a linear response of 0 to 40 mg/L BOD and the linearity is perfect(r=0.999).The response-time is 8 minutes and the recovery time less than 10 minutes.The sensor shows stability within 60 days without any decrease in signal.The standard deviation of continuous measurements (n=7) is within 5％.Storage capacity of sensors is more than 4 months by present experiments.The sensor can gives stable signals in a range of pH from 6.5 to 10.5 and the best working temperature is 35℃.The salt concentration does not affect measure signals obviously until NaCl is 80g/L in samples.Sensors show to be capable for monitoring low concentration BOD in seawater.
Microbial biosensor Bacillus licheniformis BOD Seawater measurements
Cui Jiansheng Wang Xiaohui Wang Gaizhen Ma Li
Dept.Environmental Science Hebei University of Science and Technology Shijiazhuang,China 050018 School of Public Health Hebei Medical University Shijiazhuang,China 050017
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）