Aquaporin-1 Activity of Plasma Membrane Affects MCF-7 Mammary Carcinoma Cell Migration
Recent studies uncovered an important role of aquaporins in cell migration, and migration-associated cell function such as angiogenesis, wound healing, and neutrophil motility. Here we provide evidence that aquaporin expression is involved in MCF-7 human mammary carcinoma cell migration. Reverse Transcription-Polymerase Chain Reaction, Immunoblot and Immunofluorescence analysis demonstrated two populations of MCF-7 cell clones with low (MCF-7l) and high (MCF-7h) AQP1 expression and the AQP1 protein expression patterns in the plasma membrane of MCF-7 cells. MCF-7 cell clones (MCF-7l and MCF-7h) with low and about two-fold higher osmotic water permeability were identified by functional assays with corresponding low and high AQP1 expression. Cell migration rate was remarkably higher in MCF-7h cells as compared to MCF-7l cells, assessed by wound healing and Boyden chamber assays. Adenoviral-mediated AQP1 expression in MCF-7l cells increased their water permeability and migration rate to the level similar to MCF-7h cells. These results provide the direct evidence that aquaporin-mediated membrane water permeability enhances MCF-7 mammary carcinoma cell migration and may be associated with mammary carcinoma invasion and metastasis.
aquaporin mammary carcinoma cell migration water permeability
Yong Jiang Xin Zhang Rui Su Nan Wang
Key Laboratory of Industrial Microbiology,Ministry of Education,College of Biotechnology,Tianjin University of Science and Technology Tianjin,China
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）