Establishment of the callus culture of Elaeagnus angustifolia to produce proanthocyanidins and effect of UV-B radiation on its accumulation
Callus culture of E laeagnus angustifolia was established with the objective to produce proanthocyanidins. The effects of different conditions on the callus growth and the production of proanthocyanidins were researched. The leaf tissue part of Elaeagnus angustifolia was optimum explant of callus induction. The best callus growth and the highest proanthocyanidins production was obtained under the culture on MS basal medium containing 4.50 μmol·L-1 2, 4-D and 2.20 μmol·L-1 BA. The optimum time of subculture was 20-25 d. The content of proanthocyanidins in callus was up to 56.25 mg·g-1 DW. Callus bud differentiation exhibited a negative correlation with proanthocyanins accumulation, UV-B radiation had remarkable promoting effects on the accumulation of proanthocyanidins in the callus of Elaeagnus angustifolia.
Elaeagnus angustifolia L callus proanthocyanidins
Fan-Suo Zeng Wei-Wei Wang Ya-Guang Zhan Ji-Yuan Song Xiang-Ling You Ying Xin
Key Laboratory of Forest Tree Genetic Improvement and Biotechnology,Ministry of Education,Harbin 150 Key Laboratory of Forest Tree Genetic Improvement and Biotechnology,Ministry of Education,Harbin 150 College of Life Science,Northeast Forestry University,Harbin 150040,China College of Forestry,Northeast Forestry University,Harbin 150040,China
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）