The fermentation conditions of β-carotene by the red yeast mutant RG-6p
To optimize the β-carotene fermentation medium of the mutant RG-6p (derived from Rhodotorula glutinis RG-6 with high hydrostatic pressure treatment), a response surface methodology (RSM) approach was used in conjunction with a factorial design and a central composite design. The maximum yield of β-carotene (13.4 mg/L) was obtained at a pH of 6.7 with an optimum medium (40 mL/250 mL) of yeast extract (4.23 g/L), glucose (12.11 g/L), inoculum (30 mL/L), tomato extract (2.5 mL/L), peanut oil (0.5 mL/L), and (NH4)2SO4 (5 g/L). Validity of the RSM approach was also confirmed by experiments.
β-carotene fermentation medium optimization response surface methodology
Sui-Lou Wang De-jing Chen Guo-Hong Qi Gui Tang Chen Bo Li Zhi-Ping Yang
Department of Food Science and Safety China Pharmaceutical University Nanjing 210009,Jiangsu,China S Shaanxi Key Laboratory for Resource Biology Shaanxi University of Science and Technology Hanzhong 72 Department of Food Science and Safety China Pharmaceutical University Nanjing 210009,Jiangsu,China
国际会议
北京
英文
1-3
2009-06-11(万方平台首次上网日期,不代表论文的发表时间)