Isolation and characterization of dimethoate degrading phytopathogen fungus from soil
Lots of dimethoate are applied annually in world with the remainder released to environment has brought terrible contamination. Microorganisms play an important role in the biodegradation of pesticide residues in nature. Two fungal strains with dimethoate degradation capability were isolated and named as F1 and F2 after gradual adaptation and enrichment from soil. The further tests were carried out to confirm the biodegradation activity of fungal strains on dimethoate by HPLC. Results showed that strain F1 exhibited more than five times biodegrading activity as high as strain F2 in 100 mg/L dimethoate and nineteen times in 200 mg/L dimethoate and more tolerant in 400 mg/L dimethoate. The strain F1 was capable of degrade 100 mg/L dimethoate completely in 7 days without any other carbon source supplements, but the degradation capability was weaken with the decrease of complexity of carbon source supplements. It seems that strain F1 held highest capability of dimethoate degradation in the condition of hunger. This strain was characterized and identified as Fusarium sp. by ITS sequence analysis. Fusarium is a well-known and widely distributed soil-borne phytopathogen fungus. As well as its damage to agricultural production, Fusarium also hold potential application in environment protection, so we should exploit it reasonable.
phytopathogen fungus dimethoate degradation insecticide
Jiehong Zhao Degang Zhao Jie Han
Key Laboratory of Green Pesticide and Agricultural Bioengineering of Education Ministry Guizhou Univ Guizhou Key Laboratory of Agricultural Bioengineering Guizhou University Guiyang,China Microbiology Laboratory of Foundation Department Guiyang College of Traditional Chinese Medicine Gui
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）