Potential roles of ROS and NF-κB in TNF-α release in rat alveolar macrophages ezposed to single-walled carbon nanotubes
The pulmonary toxicology of carbon nanotubes (CNTs) was highlighted recently when single-walled carbon nanotubes (SWCNTs) were reported to be highly toxic and fibrotic in mice and in rats when administered by intratracheal instillation. Therefore, it is necessary to evaluate the effects of SWCNTs on human health and the environment before utilizing them on a large scale. The purpose of this study was designed to assess the effects of SWCNTs on tumor necrosis factor-α (TNF-α) release and to elucidate possible molecular mechanisms in primary rat alveolar macrophages as a model system. Our results showed exposure of the cultured alveolar macrophages to SWCNTs elicited a concentration-dependent TNF-α release and reactive oxygen species (ROS) production in alveolar macrophages. In the presence of the antioxidants N-acetyl-Lcysteine (NAC) and glutathione (GSH), the SWCNTs-induced of TNF-α release and ROS production was significantly reduced. SWCNTs treatment caused an activation of the redox-sensitive transcription factor nuclear factor-kappaB (NF-κB). Pretreatment of alveolar macrophages with NF-κB inhibitors, L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK) and parthenolide, suppressed SWCNTs-induced TNF-α expression. These results indicate that SWCNTs are able to induce TNF-α release in rat primary alveolar macrophages, at least in part, mediated by oxidative stress and NF-κB activation.
Single-walled carbon nanotubes Reactive ozygen species Tumor necrosis factor-α NF-kappaB
Shefang Ye Yihui Wu Kai Wei Liming Zhong
Research Center of Biomedical Engineering;Research Center of Biomedical Engineering Technology of Xiamen City,Key Laboratory of Biomedical Engineering of Fujian Province;Department of Biomaterials,College of Materials,Xiamen University,Xiamen,P.R.China In
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）