Production of a novel bioflocculant by culture of Pseudomonas fluorescensC-2 using molasses wastewater
In this study, the optimal conditions of production of bioflocculant by the culture of Pseudomonas fluorescens C-2 were examined, using molasses wastewater to replace glucose as carbon source and energy source in the culture medium. Results showed that the COD concentration in molasses wastewater favorable for the production of the bioflocculant PS-2 was 8000 mg·L-1, and an optimal culture condition of inoculum size of 5％(v/v), 30C, initial pH 8.0 and shaking speed of 160 r·min-1, under the optimal culture conditions, the highest flocculating activity achieved for Kaolin suspension was 92.5％ and 2.52 g pure PS-2 per liter broth was obtained after 72 h cultivation. The wastewater treatment experiments results indicated the chromaticity removal efficiencies of the bioflocculant PS-2 for breed aquatics wastewater and domestic sewage were above 91％ with a PS-2 dosage of 120 mL·L-1, the turbidity removal efficiencies of the PS-2 for four wastewaters were all above 90％ with a bioflocculant PS-2 dosage of 120 mL·L-1.The bioflocculant PS-2 produced usIn this study, the optimal conditions of production of bioflocculant by the culture of Pseudomonas fluorescens C-2 were examined, using molasses wastewater to replace glucose as carbon source and energy source in the culture medium. Results showed that the COD concentration in molasses wastewater favorable for the production of the bioflocculant PS-2 was 8000 mg·L-1, and an optimal culture condition of inoculum size of 5％(v/v), 30C, initial pH 8.0 and shaking speed of 160 r·min-1, under the optimal culture conditions, the highest flocculating activity achieved for Kaolin suspension was 92.5％ and 2.52 g pure PS-2 per liter broth was obtained after 72 h cultivation. The wastewater treatment experiments results indicated the chromaticity removal efficiencies of the bioflocculant PS-2 for breed aquatics wastewater and domestic sewage were above 91％ with a PS-2 dosage of 120 mL·L-1, the turbidity removal efficiencies of the PS-2 for four wastewaters were all above 90％ with a bioflocculant PS-2 dosage of 120 mL·L-1.The bioflocculant PS-2 produced using molasses wastewater substrate possesses flocculating activity comparable or superior to that of bioflocculant produced using conventional substratefing molasses wastewater substrate possesses flocculating activity comparable or superior to that of bioflocculant produced using conventional substrate.
Pseudomonas fluorescens Bioflocculant Molasses wastewater Removal of chromaticity and turbidity
Yan-Li Mao Ling-Xian Luo Rui-Qun Liu Kui Zhang Yao-Qing Wang Yong-Sheng Yan
School of Chemistry and Chemical Engineering Jiangsu University Zhenjiang,Jiangsu,212013 Henan Unive Henan University of Urban Construction Pingdingshan,Henan,467001 Construction Engineering Quality Supervise Station of Pingdingshan Construction Commmittee Pingdings Henan Fifth Construction and Installation Project Limited Company,Pingdingshan,Henan,467001 School of Chemistry and Chemical Engineering Jiangsu University Zhenjiang,Jiangsu,212013
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）