A Rapid Quantitative Detection Method for anaerobic denitrifying bacteria(ADNB)
There was a long detection circle and higher cost in the quantitative detection of anaerobic deitrifying bacteria(ADNB) in the wastewater of bioreactor and oil field. To solve these issues, the ADNB-MPN-PCR method, combining polymerase chain reaction(PCR) technology with most probable number, was studied as a rapid quantitative detection method for ADNB. The bacterium solution directly used for PCR amplification was prepared from wastewater, which ensured the accuracy of quantification; the universal primers, ADNB341 and ADNB870, located at 16S rDNA genes of ADNB were designed, and the amplified fragment was 548bp; the reaction system and amplification conditions of PCR were also optimized. The results indicate that the order of magnitude of this method is two magnitudes higher than that of MPN-Gries, which truly represents the actual quantity of ADNB in wastewater. The whole procedure takes only 3～4 hours and the results of the detection are extremely stable, so the cost is reduced and ADNB-MPN-PCR can be applied in practical processes.
Anaerobic deitrifying bacteria Bacterium solution preparation Quantitative detection 16S rDNA ADNB-MPN- PCR assay
Li WEI Ji-xian Yang Yan-jun Wang Fang Ma Qiang Wang Xiao-lei LV
State Key Laboratory of Urban Water Resources and Environment,Harbin Institute of Technology,Harbin, Department of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China;Depart
国际会议
北京
英文
1-4
2009-06-11（万方平台首次上网日期，不代表论文的发表时间）