HETEROZYGOTE DETECTION BY COLOR-CODED MINISEQUENCING
Dual-color method was applied in solid phase minisequencing to genotyping SNP, including heterozygote with 2 alleles. But this method could not genotype heterozygote with over 2 alleles. In this study, we introduced color-coded method to solid phase minisequencing to genotype heterozygote with over 2 alleles. In our experiment, 4 templates with 4 different alleles were synthesized. Cy3 - ddUTP, Cy3 - ddCTP, Cy5 - ddUTP, and Cy5 - ddGTP were mixed as the color-coded ddNTPs in our minisequencing experiment. The on-chip extension was performed subsequently with the color-coded ddNTPs. As what we anticipated, the signals of the six templates mixtures in Cy3 and Cy5 channel exhibit evident differences between each other and the results demonstrated the feasibility of 2 - color solid phase minisequencing in heterozygote genotyping.
Minisequencing color-coded heterozygote
Tu Jing Yang Qi Bai Yunfei Sun Beili Ge Qinyu Lu Zuhong
State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China Key laboratory of Child Development and Learning Science, Ministry of Education,Southeast University State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China Key laboratory o
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
79-83
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）