CONSTRUCTION OF ONLINE CONTINUOUS CHROMATOGRAPHY SYSTEM USING MONOLITHIC COLUMN FOR SINGLE CELL ANALYSIS
Demands on speedy chromatographic separations of bio-related substances without loss of samples are increasing in the fields of proteomics. In addition, the system with sensitive detection of cellular components without loss of samples has been required. The online chromatography system including the invasive disruption of cells, extraction of cellular contents, and their separation, is appreciated for this purpose. In the first step, we examined a cell disruption method with a femto-second laser. The analyte extracted from a living cell is load on a monolithic cartridge directly for the chromatographic separation method. The monolithic column has been developed as a speedy separation medium with a low-pressure drop and exhibits the potential for high efficient separations. We have developed these analytical methods with offers of various choices of analytical systems according to the samples and purposes.
Protein separation 2D- HPLC monolithic column titania column single cell analysis proteome analysis
Hironobu Morisaka Keisuke Hara Mitsuyoshi Ueda
Laboratory of Biomacromolecular Chemistry, Division of Applied Life Sciences, Graduate School of Agr Laboratory of Biomacromolecular Chemistry, Division of Applied Life Sciences,Graduate School of Agri Laboratory of Biomacromolecular Chemistry, Division of Applied Life Sciences, Graduate School of Agr
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
249-250
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）