Production of High Optical Purity L-lactic Acid with Fiber Wastes from NaOH-AQ Pulping System

摘要：

This study proposed a novel bioprocess utilization of fiber wastes from pulping process for the production of L-lactic acid.The bioprocess of Respective Saccharification Fermentation(RSF)and Simultaneous Saccharification and Fermentation(SSF)for L-lactic acid production by Bacillus coagulans using screened fiber wastes from a NaOH-AQ pulping system was investigated.The results showed the suitable conditions during RSF were:enzyme dosage 25IU/g,enzymatic hydrolysis time 72 h,enzymatic temperature 50℃,pH 4.8,inoculation size 10％,ferment time 48h,ferment temperature 37℃.Under these conditions,L-lactic acid content was up to 28.5 mg/ml and the purity was up to 96.4％.The optimum conditions during SSF were:enzyme dosage 30 IU/g,enzymatic temperature 50℃,pH 4.8,inoculation size 12％,ferment temperature 43℃,fermentation time 72h.Under these conditions,the reducing sugar was up to 25.6mg/ml,L-lactic acid content was up to33.5 mg/mL and the purity was up to 96.8％.

关键词： fiber waste SSF Bacillus coagulans L-lactic acid

作者: CHANG LIMIN LU JIE ZHAO CHANGXIN

作者单位: Dalian Polytechnic University,Dalian,China Dalian Polytechnic University,Dalian,China;State Key Laboratory of Pulp and Paper Engineering,South

会议类型: 国际会议

会议名称: 第二届国际造纸与环境学术会议(The Second International Papermaking & Environment Conference)

会议地点: 天津

会议语种:英文

页码: 150-153

在线出版日期: 2008-05-14（万方平台首次上网日期，不代表论文的发表时间）