Preparation of CNTs/HAp/Chitosan hydrogel and Its Cellular Compatibility
This study is made to prepare CNTS/HAp/CS thermosensitive hydrogel and detect its cytotoxicity according to the national standards in order to provide a new and effective thermosensitive hydrogel for tumor thermotherapy for clinical practice. Firstly, Nano-HAP powder prepared through a chemical precipitation process is mixed with CNTS in a mass ratio of 1:1 to obtain Hap-coated CNTs by 6h ball milling under wet conditions at the rate of 300r/min,which is then surface modified by chitosan. The chitosan- Sodium glycerophosphas is added with citric acid of certain content to get low-pH thermosensitive hydrogel which is uniformly mixed with HA/CNTs powder by some specific technique to acquire CNTS/HAp/CS thermosensitive hydrogel. Secondly, four L929 cell-culture plates are respectively put into 100μL extract solutions of different concentrations(at 100％、75％、50％ and 25％) for 72hours. The density and morphology of cells are observed, RCGs are detected with MTT assay and cytotoxicities of materials are measured. And finally, 3mm-diameter disks processed by thin film materials of low temperature and drying are co-cultured with L929 for 72, 120 hours to prepare electron microscopic samples. The relationship between cell morphology and materials are studied. The cellular toxicity of prepared materials range from 0 to 1 degree with little effect on proliferation and morphology of cultured cells. The results show this material has good cellular compatibility as biomedical material.
carbon nanotubes(CNTs) Nano-hydroxyapatite(Hap) Nanocomposites biocompatibility CNTs/HAp/CS hydrogel
杨加峰 孙昌 王兰蕾 孙康宁 刘爱红 邱丽萍 宗宪磊 李爱民 朱婧 魏源
山东大学材料液态结构及其遗传性教育部重点实验室,中国,济南250061;山东省工程陶瓷重点实验室,中国,济南250061;山东大学第二医院整形外科,中国,济南250033 山东大学材料液态结构及其遗传性教育部重点实验室,中国,济南250061;山东省工程陶瓷重点实验室,中国,济南250061 四川大学华西口腔医学院,中国,成都 山东大学第二医院整形外科,中国,济南250033
国际会议
成都
英文
2007-11-19（万方平台首次上网日期，不代表论文的发表时间）