Let7b represses the proportion of CSCs in liver cancer and the migration of liver cancer cell via inhibit the Wnt/β-catenin pathway
Let7b miRNAs are involved in carcinogenesis and tumor progression through their roles in maintaining differentiation and normal development.However, there is little research focusing on the effects of Let7b on Wnt-activated stemness of liver cancer stem cells(CSCs).To study this, we cultured human liver cancer cells HUH7 and MHCC97H ,enhanced or inhibited the expression of let7b in two cell-lines, measured the wnt signaling related protein β-catenin and frizzled (FZD) family receptor, observed the CD133+ CSCs proportion in liver cancer cell lines.We found that enhanced expression of Let7b decreased the expression of β-catenin and FZD4, inversely inhibited Let7b expression increased β-catenin and FZD4 expressions.Enhanced Let7b expression reduced the proportion of CSCs in liver cancer cell lines meanwhile Let7b inhibition increased the proportion of CSCs.Up regulated Let7b expression repressed the proliferation and migration of liver cancer cells.Our study showed the Let7b inhibite not only the migration and proliferation of liver cancer cell but also the proportion of CSCs in liver cancer by regulatingwnt/β-catenin signaling pathway.
Liver cancer Let7b Stem cell Wnt/β-catenin pathway
Yifeng Chen Hui Cai Xiaojun Yang Haizhong Ma Shixun Ma Xiangdong Niu Yupeng Zhang
General surgery clinical center of Gansu province, Lanzhou, 730000, China
国内会议
兰州
英文
553-559
2016-10-01（万方平台首次上网日期，不代表论文的发表时间）