Molecular cloning,recombinant protein expression,tissue distribution and functional analysis of a new c-type lysozyme from Lezhi black goat rumen
Three major distinct types of lysozymes have been identified in the animal kingdom and most lysozymes cloned from ruminants belong to the chicken-type(c-type).In this study,a new c-type lysozyme gene was cloned and sequenced,named LZRLyz,from the Lezhi black goat rumen.The LZRLyz cDNA has a 444 bp open reading frame(ORF)encoding a 147 amino acid polypeptide.The encoded polypeptide is predicted to have an 18 amino acid signal peptide,and a 129 amino acid mature protein with an isoelectric point(pI)of 6.08.The LZRLyz amino acid sequence shares 70.27%identity with the capra hircus blood lysozyme and is grouped with other ruminants c-type lysozymes using the phylogenetic tree estimated by Neighbor-Jointing method.The recombinant expressed LZRLyz protein(pET-rLZR)shows a molecular mass of~33 kDa which is consistent with the predicted fusion protein molecular mass and shows antimicrobial activity.Quantitative real-time RT-PCR analyses revealed that LZRLyz transcripts are expressed in all tested tissues with the predominant expression being observed in rumen and the weakest one in spleen.Results of this study suggested that the LZRLyz gene represents a new c-type lysozyme gene that likely functions in Lezhi black goat host immunity and digestive systems.
C-type lysozyme Lezhi black goat recombinant protein expression antimicrobial activity immunity gene expression
Peng Zhang Yong Wang Mingfeng Jiang Hongning Wang
Key Laboratory of Sichuan Province for Qinghai-Tibetan Plateau Animal Genetic Resource Reservation a School of Life Science,Sichuan University,Animal Disease Prevention and Food Safety Key Laboratory o
国内会议
重庆
英文
1602-1614
2016-05-25（万方平台首次上网日期，不代表论文的发表时间）