Protection Effects of Tranilast on Oxazolone-Induced Rat Colitis through a Mast Cell Dependent Pathway
Background: Mast cells in the gut play an important role in the innate and adaptive immune responses which are very relevant to human inflammatory bowel disease.However, the contribution of mast cells to the development of inflammatory, bowel disease is not well understood.Aims: This study aimed to determine the role of mast cells in oxazolone induced colitis and to explore whether the mast cell membrane stabilizer tranilast could ameliorate this colonic inflammation.Methods: Wild-type rats and mast cell deficient rats were sensitized and challenged with oxazolone.And then treated with tranilast after challenging.Controls were treated with saline.Results:Mast cell deficient rats presented weak response to oxazolone, while wild-type rats showed severe ulcerative colitis after stimulation with oxazolone.The mast cell deficient rats model (1.8±1.64) had significantly lower disease activity index score than that of wild-type rats (8.3±0.58), P<0.01.Tranilast could reduce the secretion of cytokines, immunoglobulins and myeloperoxidase activity in tranilast treatment groups compared with the model group.The number of mast cells in wild-type model was higher than other groups.There was no significant change in mast cell deficient rats.Conclusion:Mast cells play an important role in oxazolone-induced colitis.Mast cell membrane stabilizer tranilast can ameliorate oxazolone-induced colitis through a mast cell dependent pathway.
Mast cell Ulcerative colitis Tranilast IL-33
HongqianChua Jun Li Hongpeng Huang Qiuqiong Tang Siyuan Jiang Yingjuan Liu Yuan Li Ming Jin Xuexian Zhou Wanyi Wang Yuzheng Lian Yao Pan Weidong Hao Liping Duan Xuetao Wei
Department of Toxicology, School of Public Health, Peking University, Beijing, 100191, PR China;Beij Department of Gastroenterology, Peking University Third Hospital,Beijing, 100191, PR China
国内会议
河南开封
英文
58-74
2016-11-01（万方平台首次上网日期，不代表论文的发表时间）