The relationships between the chemosensitivity of human gastric cancer to paclitaxel and the expressions of class Ⅲ β-tubulin, MAPT and survivin
Lack of effective biomarkers is one of the challenges in current chemotherapy to predict drug response and sensitivity.This study was to investigate the relationships between the expressions of class Ⅲ β-tubulin, microtubule-associated protein tau (MAPT), survivin and the sensitivity of primary gastric cancer to paclitaxel treatment.Reverse transcription-PCR and Western blot were used to evaluate the mRNA and protein expressions of class Ⅲ β-tubulin, MAPT and survivin in fifty-four gastric cancer tissues.Viable tumor cells from gastric carcinomas were tested for their sensitivity to paclitaxel using adenosine triphosphate-based tumor chemosensitivity assay (ATP-TCA) in vitro.Out of 54 samples, 30 samples were sensitive to paclitaxel, while the other 24 samples were resistant.The overall efficacy of paclitaxel was 55.56% (30/54).The mRNA expressions of Class Ⅲ β-tubulin and survivin were significantly correlated to the histological grade (P=0.029, 0.009, respectively).The sensitivity of gastric cancer patients to paclitaxel treatment was inversely correlated to the mRNA and protein expressions of class Ⅲ β-tubulin (P<0.0 t), MAPT (P<0.05), and survivin (P<0.05).A significant positive correlations were found between Class Ⅲ β-tubulin and MAPT expression at mRNA and protein levels (mRNA: P=0.037; protein: P=0.001).Our results indicate that the expression levels of Class Ⅲ β-tubulin, MAPT and survivin are good biomarkers for predicting the sensitivity of gastric cancer to paclitaxel treatment.
Keywords Gastric cancer Paclitaxel Class Ⅲ β-tubulin MAPT Survivin ATP-TCA
Wenting He Dachuan Zhang Jingting Jiang Ping Liu Changping Wu
Department of Oncology,the Third Affiliated Hospital of Soochow University,185 Juqian Street,Changzh Department of Pathology,the Third Affiliated Hospital of Soochow University,185 Juqian Street,Changz Department of Oncology,the First Affiliated Hospital of Nanjing Medical University,300 Guangzhou Roa
国内会议
上海
英文
71-79
2014-06-21（万方平台首次上网日期，不代表论文的发表时间）