Determination of Content of Chlorogenic Acid and Caffeic Acid in Leontopodium leontopodioides by HPLC
Object: To establish high performance liquid chromatography (HPLC) method for determination of content of chlorogenic acid and caffeic acid in Leontopodium leontopodioides for the first time.Method: The stationary phase was GiamonsiiTM C18 (5μm, 250×4.6mm) column and the mobile phase was acetonitrile-0.5% phosphoric acid (10∶90).The flow rate was maintained at 1.0mL/min and the column oven temperature was 40℃.We set 280nm as detective wavelength.Results: The liner range of chlorogenic acid was 0.08-0.76μg (r=0.9999).The average recovery (n=5) was 99.2%.Conclusion: The method is simple, accurate and sensible.And this will provide reference for the quality control of Leontopodium leontopodioides.
Leontopodium leontopodioides chlorogenic acid caffeic acid HPLC content determination
Gong Jian Suo Fei-ya Wang Aoli Ma Yun Huang Luo Dong Li Ping Ye Xing Zhang Lin
College of Life Science and Technology, Xinjiang University, Urumqi 830046,China
国内会议
重庆
英文
294-299
2011-12-14（万方平台首次上网日期，不代表论文的发表时间）